Journal: Translational Cancer Research
Article Title: SPDEF downregulation promotes tumor aggressiveness and poor prognosis in triple-negative breast cancer
doi: 10.21037/tcr-2026-1-0130
Figure Lengend Snippet: Regulatory effects of SPDEF expression on apoptosis-related protein expression in triple-negative breast cancer cells. (A) Western blot detection of apoptosis-related protein expression in MAD-MB-468 and MAD-MB-468 cell lines. (B-D) Quantitative analysis bar graphs of pro-apoptotic protein expression: Bax (B), cleaved caspase-3 (C), and cleaved caspase-9 (D). (E,F) Quantitative analysis bar graphs of anti-apoptotic protein expression: Bcl-2 (E) and Bcl-xL (F). *, P<0.05; **, P<0.01; ***, P<0.001. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NC, negative control; OE, overexpression; SPDEF, SAM pointed domain-containing ETS transcription factor.
Article Snippet: The BCL-XL antibody (catalog number: AF6414) was purchased from Jiangsu Affinity Biosciences (Jiangsu, China).
Techniques: Expressing, Western Blot, Negative Control, Over Expression